mouse antihuman monoclonal igg1 against vcam 1  (Bio-Rad)

Journal: Mediators of Inflammation

Article Title: Molecular Responses of Human Retinal Cells to Infection with Dengue Virus

doi: 10.1155/2017/3164375

Figure Lengend Snippet: Cell adhesion molecule expression by human retinal endothelial cells infected with DENV: viral strain = Mon601; multiplicity of infection = 1; evaluated time points postinoculation = 24, 48, and 72 hours (hr). (a) Graphs showing relative transcript expression for adhesion molecules in DENV-infected versus mock-infected endothelial cells. Reference genes were glyceraldehyde-3-phosphate dehydrogenase and TATA-binding protein. Bars represent mean relative expression, with error bars showing standard deviation. n = 3 cultures/condition. Data were analyzed by two-tailed Student's t -test. (b) Graph showing relative expression of VCAM-1 protein on the surface of DENV-infected versus mock-infected endothelial cells. Each pair of circles joined by a line represents the mean expression for DENV-infected versus mock-infected endothelial cells for one of 3 independent experiments. n = 8 cultures/condition. Data were analyzed by one-tailed paired Student's t -test. ICAM-1 = intercellular adhesion molecule, 1; VCAM-1 = vascular cell adhesion molecule 1; ALCAM = activated leukocyte cell adhesion molecule.

Article Snippet: Following a 30-minute block with 5% w / v skim milk in PBS (blocking solution), the monolayers were incubated with mouse monoclonal anti-human vascular cell adhesion molecule 1 (VCAM-1) antibody (BD Pharmingen: catalogue number 555645) or mouse monoclonal IgG (BD Pharmingen) at 1 μ g/mL in blocking solution for 45 minutes at room temperature ( n = 8 monolayers/condition) and washed 5 times with PBS/Tween-20.

Techniques: Expressing, Infection, Binding Assay, Standard Deviation, Two Tailed Test, One-tailed Test